| |
|
|
 |
|
print
|
|
|
|
|
|
1. Microorganism Capture
Recover microorganisms from filterable samples using a 0.2 micron spin filter. RNA booster is added to equilibrate the ribosomal RNA pool. (Alternate protocols are available for non-filterable samples.) |
2. Cell Lysis
Resuspend microorganisms in lysis buffer, transfer to lysis tube and place in mechanical lysis system. A combination of mechanical lysis and lysis buffer breaks down cells and spores to liberate nucleic acid from the sample. |
3. RNA Extraction
After lysis, the samples are transferred to a 96-well plate. Sample and reagent plates are loaded onto the RNA Extraction Robot. RNA binds to the surface of the magnetic beads and is purified through a series of wash steps. |
4. RT-PCR Preparation
The extracted RNA sample and PCR reagents are added to capillary tubes. |
5. RT-PCR
The closed capillary tubes are placed in the carousel and loaded into the Thermal Cycler. The Thermal Cycler generates a real-time PCR report. |
|
|
|
|
|
|
|
|
 |
