What can I expect to see in a well on a 96 well plate after transfecting the CHOK1SV host cells?
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1st week post transfection
During the first week following addition of selective medium, it appears as if no cells have survived the transfection procedure; indicated by a shrunken appearance of cells.
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2nd week post transfection
During the second week, the majority of the wells still appear to contain no growing cells and colonies will not be obvious at this stage. However, if screened using a light microscope, it may be possible to observe the beginning of a colony. A small cluster of cells may be observed that show typical fibroblast morphology and appear elongated and flattened against the surface of the well and/or appear as bright, round cells.
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3rd week post transfection
Approximately three weeks after addition of selective medium, the first few colonies may begin to become visible with the naked eye when the plates are examined from underneath. A slight colour change can be observed in these wells; the medium becoming orange as the colony is growing.
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4th week post transfection (1/2)
Approximately four weeks after addition of selective medium, the first colonies will become much more distinct and should be visible with the naked eye when the plates are examined from underneath. Wells should be scanned for dense patches of cells, which may indicate the presence of a colony. Verification can be made under a light microscope. The colony should appear as a dense patch of cells (approximately 1 to 2 mm in diameter).
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4th week post transfection (2/2)
The cells in the colony may show typical fibroblast morphology and appear elongated and flattened against the surface of the well and/or appear as bright, round cells. Typically, the cells within the colony appear bright and round while those around the edge of the colony show the typical fibroblast morphology and appear quite elongated and bright. A colour change can be observed in these wells; the medium becoming yellow as the colony is growing.
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